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Used applicators can be tested by staining methods or by ultraviolet light to know if they have come in contact with the vaginal surface.
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Cell proliferation was tested by staining for BrdU incorporation using specific anti-BrdU antibody and FACS analysis.
The specificity of the polyclonal anti-plectin antibody (ab83497) used in the immunofluorescence staining was tested by staining pancreatic adenocarcinoma.
The ability of the differentiated HBM-MSCs to produce insulin was tested by staining with diphenylthiocarbazone (DTZ, zinc-chelating agent).
The biofilm producing ability of K. pneumoniae was tested by staining the heat fixed cells with crystal violet.
Specificity of all antibodies was tested by staining tissues from C. elegans that were either treated with RNAi against the antigen gene or mutant for the gene.
The possibility that histone H1 induces apoptosis was tested by staining for activated caspase 3 at 6 and 24 hrs following application of histone H1.
Proliferating cells were tested by staining of section with mouse anti-Ki-67 antibody (Neomarkers/Labvision) and angiogenesis was visualized by staining with mouse anti-CD34 antibodies (Neomarkers/Labvision, Fremont, CA, USA).
The purity of T-cells was tested by CD3 staining in Flow cytometry (>90% CD3+ purity).
In same mice, proliferation was tested by immunohistochemical staining of PCNA and vascularisation by α-actin smooth muscle staining on 5 µm thick sections from formalin-fixed, paraffin-embedded tissue blocks.
The level of ALDH1 and PCNA was tested by immunohistochemistry staining in paraffin-embedded tissue sections.
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