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Pain sensitivity can be tested by inserting a balloon into a person's back end (yup) and inflating it until they report feeling pain.
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This was tested by inserting a Tn 10dTc tetA) on an R1 plasmid in a strain with the lac mutation on the standard F′ lac plasmid.
A total of 5 nondegenerate oligonucleotide pairs were tested by inserting them into the site right after the initiate codon of ZeoR gene.
The authenticity of clone was tested by sequencing the insert and comparing the DNA sequence with NCBI data base.
The identity of all inserted fragments was tested by restriction endonuclease digestion and confirmed by sequencing.
The coated inserts were tested by dry machining of aluminum matrix composite (Al 10%Al2O3) bars.
The insert orientation was tested by size analysis of KpnI/NdeI double digestion fragments.
The presence of the insert within the vector was tested by PCR with oLC748/805.
The presence of the inserts within the vector was tested by PCR with oLC1175/274 and oLC1178/659.
Plasmids obtained from the transformants and were tested by PCR for the presence of PcNLP gene insert.
All constructs were tested by restriction enzyme digestion for the presence of the insert.
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