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The pGAPZB-EpBpI*p plasmid encoded three carotenogenic enzymes that were engineered to be targeted into peroxisomes of P. pastoris whereas the pGAPZB-EBI* plasmid encoded non-targeted enzymes.
Drugs can be targeted into African trypanosomes by exploiting carrier proteins at the surface of these parasites.
In conclusion, we first showed that carotenogenic enzymes can be targeted into the specific cellular location of recombinant hosts and this targeting strategy can serve as the basis for the subsequent development of sophisticated pathway engineering in microorganisms.
PKKKRKV (Pro-Lys-Lys-Lys-Arg-Lys-Val, PV7), a seven amino acid peptide, has emerged as one of the primary nuclear localization signals that can be targeted into cell nucleus via the nuclear import machinery.
In P. tricornutum we predict two glutaredoxins to be targeted into the plastids (43497, 39133), one to the cytosol (16854), and one to the mitochondria (37615).
In some cases the respective enzymes have been shown to be targeted into the mitochondria by fusing the presequences to GFP (C. Rio Bartulos, personal communication).
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They have been targeted into cells showing selective antiproliferative activity on tumor cells.
Mitochondria lysosome contacts formed dynamically in healthy untreated cells and were distinct from damaged mitochondria that were targeted into lysosomes for degradation6,7.
After the functional architecture is determined by optical imaging, electrodes are targeted into a selected cortical site under full visual control, at a variety of penetration angles (30 90°), accessing a large cortical area.
Anything that has to do with finance is targeted into these two groups, and they work together with one reporting to the other and then back to me in some fashion.
However, the mechanism of how these GPCRs are targeted into the caveolae is still unknown.
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