The mechanism by which such changes in ploidy occur during gene replacement is not known, but this phenomenon is an indication that the gene being targeted for replacement is essential.
Meanwhile Ferrari remained unruffled by speculation that Raikkonen would be targeted for 2007, either as a partner to Schumacher or as a replacement.
The leu3+ gene in this species was targeted for gene replacement with the KanMX6 marker, with 300 bp of DNA sequence corresponding to the 5' and 3' ends of the leu3+ gene used to target the construct to the native locus by homologous recombination.
The leu3+ gene, predicted to encode α-isopropylmalate synthase (Figure 1), was targeted for deletion using a homologous recombination replacement strategy.
Nevertheless, this new service was targeted for those who could afford user-pay/copayment services as an alternative rather than as a replacement for existing services.
So far, no tandem arrays of genes have been successfully targeted for replacement in Leishmania spp. The C. elegans Hsp90 was described as resistant to the inhibitor GA (David et al., 2003), opening the possibility to express a GA-resistant Hsp90 while blocking the endogenously encoded Hsp90 with the drug.
In light of the fact that the pipes were not targeted for replacement, city leaders quizzed the executives about whether the DWP could deploy new technology to detect which pipes are most at risk.
Real Madrid forward Alvaro Morata, 20, may be targeted by local rivals Atletico Madrid as a possible replacement for Radamel Falcao or Adrian Lopez.
After removal of tetRA, a "scar sequence" encoding the amino acid sequence MIPGIRRPAVRSSTSLGSIGTSKQLQPT plus the last six amino acids of the gene targeted for replacement is encoded in place of the original gene.
It's targeted at "friends and family" -- like a replacement for your family newsletter, they say.
