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We found a range of specialist and generalist lineages across our study region, suggesting different evolutionary strategies being used by different lineages across elevational gradients, but caution should be taken in initial interpretation.
Special care should be taken in initial classification rounds to extract and include false negatives, as low sensitivity is highly detrimental to the quality and completeness of the update.
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Care must be taken in selecting an initial set of parameter values.
First-time viewers unfamiliar with Lynch's ways will be taken in by the initial set-up: an amnesiac car-crash victim (Laura Harring) staggers into the house of an aspiring actress recently arrived in town (Naomi Watts), but three-quarters of the way through, having been drawn into a glossy noir fantasy, the rug is pulled out from under us completely.
Another CT image was taken in the initial assessment after chemotherapy.
A two-step approach was taken in which the initial reverse transcription was followed by the qRT-PCR amplification After DNase I (Invitrogen Corporation, CA) treatment, DNA-free total RNA was reverse transcribed using a dT20VN primer (Sigma, Oakville, ON) with SuperScript II.
Histological samples of the duodenum were taken in order of initial segment, middle segment and distal segment, and they were similar to jejunum and ileum.
An iterative approach was taken in which the larger initial set of items was examined through a combination of classical and IRT approaches; thus, the earlier expert review from which the ICF items had been derived provided an initial possible structure for the items and which provided the starting point for the subsequent structural analyses of the HF-ICF-60 measure.
Venous blood samples 10-20mll) were taken in EDTA tubes at the initial visit for all patients and control subjects and stored at −80°C prior to DNA extraction.
Thus, several approaches are being taken in the ACCORD trial to prevent initial episodes of severe hypoglycemia, to monitor the frequency of episodes that do occur, and to prevent recurrence.
The initial case was taken in Ireland, where Facebook's European HQ is based.
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