Here, the samples are taken immediately from the fabrication chamber to the microscope while under vacuum to prevent oxide formation.
Blood samples were taken immediately from the caudal vein by a sterile syringe, transferred in lithium-heparinized reaction tubes (Co. Sarstedt, Germany), and 4 TIU aprotinin (C. Roth, Germany) per mL blood was added.
At day 28 of age, two male birds of each pen were slaughtered and intestinal samples were taken immediately from the jejunum; midway between the point of entry of the bile ducts and Meckel's diverticulum, ileum; 10 cm proximal to the ileocecal junction were taken to evaluate the villus height, crypt depth and villus height: crypt depth ratio.
On day 24 of age, two male birds of each pen were slaughtered and intestinal samples were taken immediately from the jejunum; midway between the point of entry of the bile ducts and Meckel's diverticulum, ileum; 10 cm proximal to the ileo-cecal junction were taken to evaluate the villus height, crypt depth, and villus height:crypt depth ratio.
For example, if FTL mice are taken immediately from their home cage and their brains analyzed for βgal expression, some variation in expression of βgal is found between individual mice, in particular in cortical regions and hypothalamus.
An early acquisition centred on the abdomen and pelvis was taken immediately afterwards, from the hepatic dome to beneath the ischia.
Blood samples were taken immediately post mortem from the vena jugularis for the analysis of glucose (Vacuette® tube, FX Sodium Fluoride/Potassium oxalate, 2 ml), as well as insulin and leptin (Vacuette® tube, Z Serum Clot Activator, 9 ml).
Non-fasting blood samples were taken immediately after cervical dislocation from the aorta.
