The PCR mixture consisted of 600 nmol/liter of each primer, 200 nmol/liter probe (sequences used are given below), 5 units of AmpliTaq Gold polymerase, 200 μM each dATP, dCTP, and dGTP, 400 μM dUTP, 5.5 mM MgCl2, and 1X TaqMan buffer A containing a reference dye, to a final volume of 25 μl (all of the reagents were supplied by Perkin-Elmer Applied Biosystems).
Monoclonal antibodies against human ER α, Ki-67 antigen and p53 oncogene were supplied by Dako (DAKO, Denmark) and applied with dilutions 1 : 40, 1 : 100 and 1 : 300, respectively.
All of the remaining, applied reagents were supplied by POCh Gliwice, Poland.
Here the word silent applies only to the dialogue, which is supplied by titles.
"Can I apply for public assistance, because my job was supplied by federal money and that money didn't come?" she asked.
Antibodies were applied using an automated tissue immunostainer (Discovery module, Ventana Medical Systems, Phoenix, AZ, USA), DAB immunohistochemistry kit and bulk reagents that were supplied by the manufacturer.
Antibodies were applied using an automated tissue immunostainer (Discovery module, Ventana Medical System), 3, 3-diaminobenzidine IHC kit and bulk reagents were supplied by the manufacturer.
Applied to the case of substance, the sufficient reason for why a substance exists is supplied by the causes of that substance.
Power is supplied by several hydroelectric plants.
