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This approach may be suitable for normalizing tumor burden but not the axial invasion extent of tumors because it does not reflect axial size and spatial relationships between tumors and affected bones.
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Therefore, no single reference gene with stable expression is suitable for normalizing all expression data obtained from various experimental conditions.
We demonstrated that the spike-in system was suitable for normalizing our platform and determining the threshold for discriminating the hypoxia modulated genes.
The combination of HMBS, B2M, SDHA and GAPDH was suitable for normalizing gene expression data among five liver cancer cell lines, yielding a V4/5 value of 0.175, which is close to the cutoff value 0.15.
HMBS, GAPDH and UBC were identified to be suitable for the normalization of gene expression data among tumor tissues; whereas the combination of HMBS, B2M, SDHA and GAPDH was suitable for normalizing gene expression data among five liver cancer cell lines, namely Hep3B, HepG2, HuH7, SK-HEP-1 and SNU-182.
The highly significant correlation when the real time PCRs were normalised to either β-actin or ELF-1α shows that both these control genes are suitable for normalizing the IL-1β and IFN-γ responsive genes which are changed in expression.
In order to normalize antioxidant expression, a number of potential HKGs were chosen that had previously been shown to be suitable for thermal experiments with Symbiodinium C3 [ 82].
May not be suitable for hyperactive children.
PJs should be suitable for nighttime temperatures.
Concluding from the results of all three species, GAK and VPS4A seem to have a conserved expression stability and to be suitable candidates for normalizing RT-qPCR experiments on liver samples.
The M value of three genes is below the threshold value of 1.5, which showed that actin gene is suitable for using as the internal controls to normalize the expression of OSCA genes in rice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com