Sentence examples for be suitable for normalization from inspiring English sources
Therefore, these reference miRNAs could be suitable for normalization regardless of the ratio of tumor- versus normal cells in the sample.
When the majority of genes are differentially expressed, very few of those bins may be suitable for normalization, or the assumed fraction of non-differentially expressed genes may not hold.
A combination of TUBA and EF1 was found to be suitable for normalization of Alternaria sp. MG1 in different developmental stages, and 18S was found to be the least stable.
Apart from the expression level considerations, stability analysis based on the M values obtained in geNorm, the stability index from NormFinder and descriptive statistics produced by Bestkeeper suggested that all the six genes used in this study would be suitable for normalization of gene expression data in tissue-dependent manner.
Moreover, a reference gene with stable expression in one organism may not be suitable for normalization of gene expression in another [ 24, 25].
This implies that a reference gene with stable expression in one organism may not be suitable for normalization of gene expression in another organism [ 35, 36], or even in different experiments for the same species.
Gene expression was normalized to the expression of the reference gene β-actin (actb) because abundance of transcripts of actb did not differ significantly between the control and treatment groups, indicating that this gene was suitable for normalization (One-way ANOVA, p = 0.49).
In our situation, the spikes were suitable for normalization without further adjustments.
Whether these two genes are suitable for normalization of neuronal differentiation in other systems remains to be evaluated.
This indicates that some reference miRNAs are suitable for normalization of both frozen- and FFPE samples, which has been suggested previously [ 12].
HMBS, GAPDH and UBC were identified to be suitable for the normalization of gene expression data among tumor tissues.
