The results indicated that in brain, heart, liver, muscle, spleen and head kidney, respectively, 28S rRNA/TUB, 28S rRNA/TUB, RPL30/28S rRNA, RPL30/TUB, ELF-1A/28S rRNA and ELF-1A/TUB gene pairs were highly stable and were suitable as reference genes to study oxidative stress, while ACTB and B2M were the least stable genes in examined tissues under normoxic and/or hypoxic conditions.
This was followed by selection process undertaken to determine which of these genes were suitable as reference genes.
We tested whether the 28S ribosomal RNA of the pig fulfilled this requirement, and found that this gene was suitable as reference gene for the study of PRV infection, at least in our 6-h examination period.
Yet, stringently predicted H37Rv STRING PPI data appeared to be suitable as a reference for analysing functional associations rather than physical interactions.
This not only is important for microbiological investigations, but also might be suitable as a general reference point for other whole-cell detection methods, for example, fluorescence in situ hybridization [ 30].
Assessments of 2 independent observers were highly correlated (r = 0.99, n = 23), indicating that direct human observations were suitable as the reference method.
We consider that nanosized highly pure carbon black particles are suitable as a reference material for safety evaluation of CNTs.
Because there are a large number of such articles, we believe that many researchers will agree with the conclusion of this Review that carbon black is suitable as a reference material for safety evaluation of CNTs.
They concluded that only 1 of the 7 ("Elongation factor 1-α"; Elf1α) was suitable as an internal reference for the three stresses they examined.
