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In this case, it may be sufficient to sequence a well distributed portion of the genome in all individuals [ 29].
It might be sufficient to sequence all 3 CRISPR/ cas loci in all strains studied by Zasada (4 ) to gain some insight into their relatedness and possible spatiotemporal evolution.
Such high outputs are essential for human whole-genome sequencing but excessive for many other applications, where the same amount of data would be sufficient to sequence several samples in applications such as targeted sequencing, microbial genome sequencing, RNAseq, ChIP-seq and amplicons.
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This is sufficient to sequence five libraries simultaneously at about 65x coverage each.
In many cases, it is sufficient to sequence each mRNA sample only once.
We obtained approximately 100-300 clones per transformation and in most cases it was sufficient to sequence a single clone.
The cn- and zn-series are almost complete and the number of identified phosphorylated fragment ions is sufficient to sequence the peptide and to localize the phosphorylation site.
Positions 5, 10, 65 and 70 (as numbered in Fig. 1) are included in regions that have previously been shown together to be sufficient to confer sequence specificity to an HMG-box protein [ 23].
In theory, explains Philip Gregory, senior vice president and chief scientific officer at Sangamo BioSciences, a library of 64 zinc fingers (one for each codon) should be sufficient to target any sequence.
In other words, it may not be sufficient to know the sequence and the position of a given LINE-1 repeat to predict its expression.
We found that the aligned T. biloba sequences were 82.3% conserved (mean sequence conservation taken from a subset of 500 BLAST hits) indicating that BLAST may not be sufficient to identify some sequences.
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