The chimeric S. pombe dynein did not express well, either, but we were able to purify amounts that were sufficient for single-molecule studies.
This confirmed the successful adsorption of receptors onto the AFM tips through specific surface conjugation methods and the density of the surface coated receptors was sufficient for single molecule binding detection.
Since the amplification from the rolling circle reaction is sufficient for single molecule detection, any template molecule carried over would be a potential source of false signals.
On the basis of the study by Brasselet and Moerner(25) on the pH-dependent fluorescence characteristics of individual SNARF-1 molecules, we believe that the brightness and photostability of this probe should be sufficient for a single-molecule acidification assay in situ.
Amounts must be sufficient for 5 single gels and are slightly in excess.
Studies comparing the spatial location of the Rayleigh scattering maximum, or hot spot, with the location of the SERS intensity maximum suggest that enhancement outside of the gap region is sufficient for detection at the single-molecule limit.
The poly(I C) cleavage products contain 5′ monophosphate ends, supporting the possibility that in the context of some dsRNA molecules a single phosphate might be sufficient for activation of RIG-I.
This indicates that a single UBM can be sufficient for TLS.
The absorption at 561 nm is ~40% less, which may be sufficient for ensemble fluorescence imaging, but is not ideal for the low SNR found with single molecule detection.
A single peptide was demonstrated to be sufficient for a reliable quantification using iTRAQ.
