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In this regard, it is worth noting that Yap1 and Pap1 form different disulfide bonds depending on conditions and/or to alter the longevity of the response [43], [44] but also that a single cysteine pair could be sufficient for function of this mechanism although in this case there would be only one level of response.
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Scoring unipolar growth and secretion of Cts1 revealed consistent results leading to the conclusion that one PAM2L motif is sufficient for function, but if both are mutated functionality of Upa1 is lost.
One PAM2L motif is sufficient for function, but if both PAM2L motifs were mutated Upa1 functionality was lost.
More interestingly, the 16 N-terminal PPRs in PRG3 was sufficient for function (i) via sequence-specific RNA binding, whereas the 11 C-terminal motifs were essential for functions (ii) and (iii) by activating translation (Fujii et al. 2013).
In principle, maternal mdr49 could have been sufficient for function, explaining the lack of suppression.
From our analysis it is not clear whether the Mob domain alone or only together with the N-terminal extension is sufficient for function.
With the recent demonstration that for some mammalian lncRNAs the act of their transcription is sufficient for function [ 35], it becomes clear that there can be a number of lncRNAs acting in a similar way.
Given that the Cas6 group RAMPs are dedicated nucleases for the processing of the CRISPR transcripts (pre-crRNA) that produce the crRNAs and appear to be sufficient for this function [ 13, 14, 28], most of the other RAMPs might be involved in non-enzymatic functions in the respective Cascade complexes.
Moreover, complete knockdown of protein expression is difficult to achieve and residual protein can be sufficient for some function analogous to the situation with hypomorphic mutations [49].
Furthermore, the growth debilitation and avirulence found with RCOL-1 are not likely to have resulted just from the altered ribosomal gene cassettes, since there are mostly pure copies of C. oryzae-sativae in RCOL-1, which should be sufficient for full function.
Hence, these observations suggest that Ca2+, phospholipid and SNARE binding might not be sufficient for synaptotagmin-1 function.
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