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Quantitative analysis revealed that blood samples contain CTCs at numbers which should be sufficient for detection with the AdnaTest, given that this assay detected as few as 1 cell/ml in spiking experiments (Table 1).
For cell populations of 1 in 103 leukaemia cells or greater, we estimated that this first stage of processing would be sufficient for detection of single mutated cells.
In addition to the global measurement, with MiXO, the accumulated data over ({sim} 2 months (at 1 AU) during the quiet sun state alone will be sufficient for detection of ({sim } 1 % spatial variation for major elements (e.g. O-K, Fe-L) over the entire surface.
A power analysis then estimated that 200 patients (100 in each group) would be sufficient for detection of a statistical significance of 5% with 90% power, using a 2-sided Student t-test when the VAS SD was 20 mm.
However, for subclinical infections or from other species of birds, additional testing may be necessary to determine whether the amount of virus available in feather pulp will be sufficient for detection by virus isolation or RT-PCR.
Antipova et al. recently reported that utilization of a single well performing Affymetrix probe pair can be sufficient for detection of its corresponding target [ 16], further supporting our hypothesis.
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Therefore, this large difference of FLT allowed the automatic mode to detect RhodG FLT without fail, provided that the RhodG signal was sufficient for detection.
Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
This result also indicated that 5 μg of total RNA or 1 μg of small RNA was sufficient for detection.
The results suggest that this improved method is sensitive and efficient, with approximately 5 μg of total RNA being sufficient for detection.
Animals were monitored up to 100 days after treatment as this was sufficient for detection of metastases in previous work with animals followed up to 180 days post injection (p.i).
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