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We were interested in determining the lower limit of homology that can be successfully recombined using RD-PCR.
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Previously, the high soluble self-assembled bioengineering allophycocyanin trimer with N-terminus polyhistidine from Synechocystis sp. PCC 6803 had been successfully recombined and expressed in Escherichia coli strain.
Gaudí RSG transgenic fish have been successfully recombined over five generations.
Gaudí BBW2.1 transgenic fish have been successfully recombined for over six generations.
GaudíLxBBW transgenic fish have been successfully recombined for over three generations.
This indicated that both Bim fl alleles had been successfully recombined in Bim fl/fl /Vav-CreER hematopoietic cells of tamoxifen-treated mice, explaining why the BIM protein was no longer present in these cells.
Adleman (1994) shows how the problem can be coded using strands of DNA which can then be spliced and recombined using different chemical reactions.
The obtained amplicon was recombined using the Gateway BP reaction into the pDONR207 vector (Invitrogen®) and the insert was sequenced.
pLR305 and pBL262 were recombined using Gateway LR clonase II to generate pBL270.
All putative genes were successfully cloned using this approach, then subsequently recombined into Gateway® adapted destination vector pYSKH3 for A. tumefaciens mediated transformation of Z. tritici.
RGBNNT was successfully synthesized using functionalized BNNTs.
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