Typical CARS systems based on optical parametric oscillators [ 5, 6] or synchronized Ti Sapphire lasers [ 7, 8] have been successful for imaging lipids, myelin and water [ 4, 9] (for a review see [ 10]) but their relatively slow tunability has hampered their use in spectroscopy.
Although this approach was successful for imaging mitochondrial calcium transients elicited by motor axon stimulation in both WT and dP32 EC1 at 20°, robust calcium transients could not be observed in either genotype when the temperature was increased to the restrictive temperatures of 33° or 36°.
Broadband methods based on transform-limited ultrashort pulses [ 11– 13] or continuum generation [ 14] have been successful for spectral imaging of optically thin systems.
Use of ApoPep-1 conjugated with fluorescent dye or isotopes has been successful for in vivo imaging of apoptosis in tumor cells and neurons [ 38, 42, 43].
Although we believe that internalizing antibodies are likely to be more successful for imaging when labeled with residualizing radionuclides rather than with non-residualizing halogens such as iodine, because of the eventual release of radioiodine after internalization [35, 36], we do note that I-124-labeled antibodies that internalize have been used successfully for tumor imaging [6, 37].
CLAHE was originally developed for medical imaging and has been successful for the enhancement of portal images [60].
However, BLI was not successful for imaging C. albicans infection from deeper infection sites due to yet not fully understood reasons (Enjalbert et al., 2009).
We have not been successful at imaging the complex between Unc45bFlag and Hsp90.
Although several potential mGluR1 PET radioligands have been described [2 8], only two [8, 9] have proven successful for imaging nonhuman primates, and none have been tested in humans.
We also give a summary of steps leading to a successful experiment that might be designed for imaging the ultrastructures of photoreceptors, the expression of two or more different fluorescently tagged proteins, their localization, distribution, or protein dynamics within photoreceptors.
