We are also unable to conclusively exclude a role for Ubp2 at the plasma membrane, as there might have been subtle defects in ubp2Δ cells at the internalization step of endocytosis that remained undetected in our experimental conditions.
Although we did not observe gross morphological defects in the antennal and optic lobes in dmPsGEFΔ 21 flies, it is possible that there were subtle defects in these brain regions.
Minor anomalies are subtle defects of appearance and structure evaluated subjectively or by measurement.
Even though there was no obvious defect in apico-basal cell polarity in N1-KD and N4-KD cell lines when grown as monolayers over 7 days, we sought to determine whether there were more subtle defects in the establishment of cell polarity by performing a calcium switch experiment.
- Rather than being caused by a few highly penetrant mutations, neuropsychiatric disorders appear to be associated with subtle defects in numerous genes, each conferring a small increase in disease risk.
Haplo-insufficiency of its Scc2 subunit (Nipbl) causes Cornelia de Lange syndrome in humans, an effect thought but not yet proven to be due to subtle defects in cohesin's genomic distribution.
The depletion of ARP2, LET-502, MEL-11, UNC-60b or ATN-1 did lead to a small increase in the number of invaginations, suggesting that invaginations are sensitive to subtle defects in the acto-myosin cortex.
Thus, the possibility remains that there is a subtle defect in the UNG-Mre11-pol η pathway (Fig. 4).
Our data do not definitively determine the cause of the respiratory defect in the absence of MICOS, Our data do not definitively determine the cause of the respiratory defect in the absence of MICOS, beyond noting that there is a subtle defect in complex IV activity.
First, it is formally possible that there are, indeed, real, but subtle defects in the efficiency and timing at chromosomal origins that are difficult to detect by current methodologies.
