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The samples were subjected to ELISA for IL-2 concentration.
Results of any sample are subject to sampling variation.
In this study, all effluent samples were subjected to pretreatment by adsorption/flocculation/filtration process.
Protein samples were subjected to electrophoresis by 10% SDS-PAGE according to Sambrook et al [35].
All the samples were subjected to sterilization by autoclave.
In parallel, samples were subjected to analysis by mass spectrometry.
Supernatant samples and mitochondrial pellet samples were subjected to protein separation by SDS-PAGE.
The reaction was stopped by the addition of SDS sample buffer and the products were subjected to SDS-PAGE, followed by a CBB staining.
They were subjected to torture by adults.
All prepared mince samples were subjected to following analyses: All samples were subjected to pH measurement as described by Benjakul et al. ([1997]).
The treated samples were subjected to shelf life study by storing them at two different temperatures.
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