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Total RNA to be subjected to reverse transcription was extracted from tumour specimens.
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The walls were subjected to reverse cyclic displacements to failure.
The mRNA was subjected to reverse transcription to obtain single-stranded cDNA following the manufacturer's guide.
The specimen was subjected to reverse cyclic loading under controlled deformation at the tip of the beam until failure.
Then, Petroleum ether Acetone (5:1) part was subjected to reverse silica gel CC, eluting with H2O MeOH (45:55, 35:65, 15:85) to give three fractions (A5, B5, C5).
Petroleum ether:Acetone (1 2) part was subjected to reverse silica gel CC, eluting with H2O MeOH (55:45 to 0 100), to give fractions A1, B1, C1, D1, and E1.
The active Fraction-1 was subjected to reverse phase silica (C-18) cartridge, strata™ from Phenomenex and eluted with water: acetonitrile mixture in gradient mode.
Equal amount of total RNA was subjected to reverse transcription using SuperScript III First-Strand Synthesis kit (Invitrogen, 18080-051) as instructed.
This article describes the experimental results of ten partially grouted reinforced concrete masonry shear walls (PG-RCMSW) that were subjected to reverse lateral in-plane cyclic loads.
Total ribonucleic acid extracted from these samples was subjected to reverse transcription and two separate sets of multiple polymerase chain reaction.
Subfraction B5 was subjected to reverse phase column chromatography eluting with MeOH/H2O (40 60 → 60 40) followed by Sephadex LH-20 (acetone) column chromatography to yield 1 (3.5 mg) and 2 (1.8 mg).
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