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The TS that best matched the intrinsic KIEs was subject to additional analysis using polarizable continuum models (PCM) with dielectric constants of water or acetone as solvents.
For 100 putative Wnt up-regulated genes identified by microarray analysis, qPCR validation was performed on a minimum of two biological replicates; genes that showed preliminary validation (including all 22 class I and II genes) were subject to additional analysis of independent biological replicates for a minimum of three per gene.
The samples showing intermediate expression (scored 2+) were subjected to additional analysis of HER2 gene copy number by using FISH.
Vallese responds that an item like a lead-contaminated vinyl shoe, which could possibly be harmful if swallowed, might be subject to additional risk analysis.
It will also be subject to additional studies and recommendations.
Officials said she could be subject to "additional investigative action".
This analysis was subject to additional limitations.
Analysis of tissue RNA transcripts, such as in situ hybridization, is less well established and is subject to additional constraints.
Buses are subject to additional restrictions.
Second, the membrane is subject to additional selectivity requirements.
Groups are subject to additional cleanup charges if deemed necessary.
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