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In addition to facilitating the study of host and viral genetics, this platform also enables the dissection of complex host/pathogen interactions and validation of biomarkers of HBV infection, treatment responses, or potential curative therapeutic interventions using PHH/KC co-cultures, which cannot be studied using other in vitro or even in vivo xenograft systems.
In addition, epigenetic alterations, DNA methylation change and histone modifications can be studied using other sequencing approaches including Bisulfite-Seq and ChIP-seq.
In addition, the exact impact of the microbial presence on the host can be studied using other '-omic' approaches, providing a multifaceted view by combining metagenomics with metatranscriptomics, metaproteomics or metabolomics (Raes and Bork 2009; Holmes et al. 2012).
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Ability of irrigation and drainage systems was studied using other eight indices with more limited information.
Meanwhile, the human methylome has been studied using other reduced representation strategies.
The measurement of single-cell expression offers much potential and has been studied using other methods, such as the dynamic proteomics approach [ 30] where coding sequences are randomly fused to an EYPF cassette in their endogenous context.
The surface-formed films have been studied using infrared, ESCA, and other methods.
Effects of the study can therefore not be compared to studies using other kinds of samples.
Therefore, the findings should be interpreted with caution until they are validated in studies using other samples.
To the best of our knowledge, these observations have not been described in studies using other in vivo microscopy techniques.
These effects are consistent with previous studies using other chemical agents for sterilization [ 9, 42].
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