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In particular, platforms enabling live-cell imaging allow the dynamics of select chromatin regions and nuclear features to be studied in real time (seconds to hours).
Developments in fluorescence have allowed the architecture and dynamics of proteins to be studied in real time and in a cellular context with great accuracy.
Tissue renewal in the native human colonic epithelium can be studied in real time ex vivo.
This technique has the advantage that it enables studies in live cells and the influence of ligand or other modulators can be studied in real time.
Recently we showed that electron beam irradiation of self-spreading polymer-encapsulated precious metal complexes can generate in situ-doped graphenic surfaces on which the dynamics of single metal atoms can be studied in real time using an aberration-corrected high resolution electron microscope.
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Cylindrical symmetry of structures grown by droplet epitaxy is assumed in the simulations which reproduce the main features of the experimental MEM image contrast, demonstrating that droplet epitaxy can be studied in real-time.
Furthermore, IVF allowed human embryo development to be studied in real-time, beginning at the earliest stages of development.
This handicap can, in part, be overcome by the use of non-invasive imaging techniques which allow infection dynamics to be studied in real-time.
The overall performance of target preamplification can be studied in real-time, using SYBR Green I detection chemistry followed by melting curve analysis.
Such methods are not likely to replace the randomized clinical trial, yet they have other advantages in that large samples of patients can be studied in real-world settings using existing data.
Similarly, the role of various inflammatory molecules and blockers may be studied in real-time for elucidation of their mechanisms and development of next generation of anti-inflammatory therapeutics.
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