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The discrete-time model allows determining precisely a point at which the relaxation should be stopped by reducing the sample temperature well below the glass transition to freeze a certain curvature.
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The methylation was stopped by reducing the excess of diazomethane under a gentle stream of nitrogen.
Digestion was stopped by adding reducing sample buffer and heating to 99°C.
After incubation for 22-24h at room temperature (RT), intein activity was stopped by removing reducing agents (Mesna, DTT, cysteine) by dialysis over night at RT.
Reactions were stopped by addition of reducing sample buffer and boiling for 1 min. Recombinant EGFP was purchased from Biovision.
After 3 min, CN-Aα/B (1.7 µM) was added and incubated for 30 min. The reactions were stopped by boiling in reducing Laemmli Buffer and the proteins were resolved through 10% SDS-PAGE.
Reactions were stopped by adding LDS sample buffer with reducing agent (Invitrogen) followed by boiling.
The reaction was stopped by addition of LDS sample buffer with sample reducing agent (Invitrogen).
After incubation, the reaction was stopped by boiling for 10 min. The amount of released reducing sugars was determined with the dinitrosalicylic acid assay [ 51].
The reaction was stopped by adding 3,4-dinitrosalicylic acid (DNS) and centrifuging to remove paper debris; the reducing sugar content was then determined.
He was stopped by an immigration officer.
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