Sentence examples for be stimulated once from inspiring English sources

Accordingly, the expression of hilD, hilA, hilC, and rtsA, and consequently that of T3SS1, should be stimulated once S. enterica reaches the epithelial surface, where iron concentrations are high and those of oxygen low.

Recovery was delayed until the economy was stimulated once again, by war in 1939.

In the next step, we asked whether the neurons could tell which of the 4 channels that were stimulated, and whether that channel was stimulated once or twice.

For blocking the Gαq/11 signaling pathway, slices were stimulated once with taltirelin (33 µM, 15 s) followed by incubation with 2-APB (100 µM, Tocris) or U73122 (100 µM, Tocris) in aCSF for 30 min and afterwards a second taltirelin stimulation.

The control point near ST25 was stimulated once per subject.

Purified CD4+ T cells were stimulated once in vitro in the presence of the CEA peptides as previously described [3].

Therefore, PBMC either left untreated or depleted of CD25+ cells were stimulated once with the each peptide individually.

In some experiments PBMC were stimulated once in vitro with peptide prior to analysis as described [23] to extend the sensitivity of the assay.

To verify the activation of both IRFs during primary EBV infection and upon restimulation, monocytes were stimulated once or twice with EBV and the presence of phosphorylated forms of IRF3 and IRF7 was evaluated by immunoblotting.

Thus, PBMC from HLA-A2+, late stage cancer patients (breast cancer, melanoma and renal cell carcinoma) were stimulated once with the different peptides in vitro before examination by ELISPOT.

For tetramer stainings, PBMC from cancer patients and healthy donors as well as tumor infiltrating lymphocytes (TIL) from cancer patients were stimulated once in vitro with peptide, or analysed directly ex vivo.