For detection of resistance-activating substances in the extract, 10 μl extract containing 0.1% Tween 80 was spread on each of five test circles (ca. 10 mm in diameter) on each leaf with a glass rod.
Approximately 4μl of the fixed cell suspension was spread on each well, air dried, and dehydrated by successive passages through an ethanol series (50, 75, and 99.9% (v/v)) for 3 min each.
Soil supernatant was spread on each carbon source-supplemented basal medium (Table 1).
Briefly, 1.5 µl of lipids solution was spread on each platinum electrode.
Fresh overnight cultures of each bacterial strain were spread on each plate to cover approximately half of the plate surface.
American Type Culture Collection Escherichia coli 25922 was spread on each plate as growth control.
They were then grown until the mid-log phase (approximately 1 × 10 cells/ml) and then 175 μl were spread on each GPY plate.
Here, the dilutions used (100 μl of the dilutions 10-1 and 10-2 were spread on each plate) allowed to estimate the population of each genus by several hundreds to thousands CFU per gram of sediment.
For isolation of proteins from photoheterotrophically grown cultures, 100 μl of overnight 2 ml liquid aerobic culture was spread on each of 14 MPYE plates which then were incubated in anaerobic jars (GasPak, BD) for 48 h.
Aliquots of 12.2 μL of the gold-particles coated with DNA were spread on each of nine macrocarriers (BioRad, Hercules, CA) and used in bombardment assays using a BioRad PDS1000/He system.
