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In the presence of Pb2+, the substrate DNA strand can be specifically cleaved at the ribonucleotide site by DNAzyme to produce a new single-DNA on the interface.
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When recombinant protein containing this site is treated with TEV protease, it is specifically cleaved at the TEV site to produce native protein.
LC3 proteins are specifically cleaved at their carboxyl termini to form LC3-I, which has an exposed carboxy-terminal glycine that is conjugated to phosphatidylethanolamine to form LC3-II, which is tightly bound to the autophagosomal membranes and serves as an autophagic marker protein.
Our results show the DFK peptide to be specifically cleaved by cathepsin-B, specifically at the engineered site (Fig. 3) and within the target cells.
In the presence of EcoRI, the stem of hpDNA will be specifically cleaved by EcoRI.
After demethylation with MBD2, the duplex can be specifically cleaved using HpaII, which releases the labeled FAM from the GO surface and results in the recovery of fluorescence.
The selected double-hairpin structure resembles the splicing site in the bZIP60 transcript, which is specifically cleaved by IRE1 at a conserved sequence (CUG↓CUG) in each loop [ 4].
In the presence of MMP-2, the P1 was specifically cleaved into two fragments at a certain site between G and V, while SA/Thi/Pt/Pd/mhCeO2NS nanoprobes were bonded onto the resulting electrode surface through the inherent interaction between streptavidin and biotin derived from uncleaved P1.
Recently, it has been shown that Dicer is specifically cleaved by caspase-3 at two sites STTD and CGVD within the RNase IIIa domain during apoptosis.
P5D and P7D were specifically cleaved by active MMP-2 and all conjugates showed significantly better cell viability than doxorubicin at equivalent concentrations.
The LSLP was methylated by DNA MTase and then was specifically cleaved by DpnI endonuclease, producing a trigger strand.
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CEO of Professional Science Editing for Scientists @ prosciediting.com