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Orchid seed is as fine as dust and must be sown on a sterilized nutrient medium in flasks.
It needs to be sown on the younger branches of a healthy tree as here its root can penetrate the bark more easily.
A wild flower seed mix will be sown on the field.
aphrodite can be sown on 8 10 agar plates of 9 cm diameter.
Then from phenology simulations (Fig. 8b) we can trace back that Sahel108 must be sown on 20 February and EGMS1 must be sown on 4 March, a difference of 12 days.
This shows that understanding of the phenology of the two parents is important because not per definition the two parents must be sown on the same date to have flowering on the same date.
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In 2003, 5 seeds per genotype were sown on a 1 m row, in a one-block design with controls, on the 27th February 2003.
The seeds were sown on October 29th.
For plant materials at the mature flower stage, seeds were sown on vermiculite and Metro-Mix.
For antibiotic selection, seeds were sown on half-strength Murashige and Skoog plates.
For confocal microscope observations, plants were sown on vermiculite and Metro-Mix until plant height was 2 4 cm.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com