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For example, employees that live in the same city can be sorted using only one data point.
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Thy1.1-positive cells were sorted using CD90.1 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany).
Simple spikes were sorted using hardware window discriminators and digitized.
Cells were sorted using MoFlo sorter, purity of sorted cells exceeded 98.5%.
Second, to study the genetic impact of using sorted semen in nucleus herds, scenarios were simulated in which female-sorted semen was used only in a centralized nucleus herd, in which multiple ovulation and embryo transfer (MOET) took place.
Spike waveforms were sorted offline using OfflineSorter (Plexon Inc, TX).
Spikes were sorted offline using MClust (A.D. Redish, University of Minnesota, Twin cities, MN).
CD34+ hematopoietic progenitors were sorted magnetically using the autoMACSpro separator (Miltenyi Biotec, Bergisch Gladbach, Germany).
Neurons were manually sorted using xclust (Matthew A Wilson, MIT).
DAPI−/GFP+-positive cells were analysed and sorted using a FacsAria (BD Bioscience).
"Data is sorted and ranked using a method which focuses exclusively on building a narrative," Birnbaum adds.
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