In addition K143, T144, T166, G168, H234 and Y329 (numbered based on their position in MurE) were significant in binding substrates and/co-factors needed for the functional events in any three of the Mur enzymes.
First, it is evident that our analyses do not exclude that other motifs, so far uncharacterized, may be significant in DCC binding regions.
Thus, a simple but reasonable prediction based solely on protein sequence would be that there should be significant divergence in binding specificity between human and Drosophila TFs.
The nature and distribution of the various receptor binding sites is significant in patterns of control.
Three SP1 PWMs, V$SP1_Q2_01, V$SP1_Q4_01 and V$SP1_Q6, were significant in the previous analyses at the binding site and gene levels, so we utilized these matrices to search for other TFs that may work in conjunction with an SP1-like factor to regulate intestinal genes during iron-deficiency.
Using ToppGene the single molecular function that was significant in this same comparison was Neuropeptide receptor binding.
Hydrogen bonds predicted to be important for specificity based on structural considerations do not always turn out to be significant to binding in post facto analyses of molecular dynamics trajectories, which treat thermal averaging, solvent, and counterion effects rigorously.
The difference in response observed by addition of increasing amounts of rHA even at the initial reading was indicative that there was significant rHA binding to fetuin in a dose-dependent manner by the time the plate was placed in the reader.
As in the rhesus macaque, there was significant binding of AVP in amygdala nuclei and insular cortex of the humanized animals.
Mouse and human APOBEC1 were also able to bind to GST-DND1 but there was significant non-specific binding in the control lanes (Fig. 2c, d and e, lanes 1 & 2).
