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Then α% of the most dynamic tones will be set as dynamic tones.
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Oxidized methionine was set as dynamic modification and methylthio, N-terminal 8plex iTRAQ, and lysyl 8plex iTRAQ as fixed modifications.
Oxidized Met and dimethylation of N-terminus and of Lys were set as dynamic modifications while Cys thiomethylation was set as a fixed modification.
The light and heavy labels, ICAT-C (+227.127 Dandand ICAT-C 13C 9) ICAT-C 13C 9), were set as dynamic modifications for all Cys residues in the samples.
Search parameters included trypsin as the enzyme with 1 missed cleavage; oxidation of methionine was set as dynamic modification, while methylthio modification of cysteine and iTRAQ modification at N-terminus of the peptide and lysine were set as static modifications.
S-Carbamidomethylation at cysteine (+57.02 Da), NAPQI-induced alkylation at cysteine (+149.15 Da), and oxidation at methionine (+15.60 Da) were set as dynamic modifications to identify the spectra of adducted peptides.
Carbamidomethylation was set as static, and oxidation of methionine and phosphorylation of serine, threonine and tyrosine as dynamic modification respectively.
Oxidation (M) was set as the dynamic modification; carbamidomethylation (C) was used as the static modification.
The femoral model was set as a dynamic rigid body, and the two planes were set as a static rigid body.
Search parameters included trypsin as the enzyme with maximum 1 missed cleavage allowed; oxidation of methionine was set as a dynamic modification while alkylation at cysteine and iTRAQ modification at N-terminus of the peptide and lysine were set as static modifications.
The solution viscosity was set as 0.8872 cP, which is the dynamic viscosity of water at 25 °C.
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