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Using different approaches, massively parallel sequencing methods overcome the limited scalability of traditional Sanger sequencing by either creating micro-reactors and/or attaching the DNA molecules to be sequenced to solid surfaces or beads, allowing for millions of sequencing reactions to happen in parallel.
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Two additional breast tumors were sequenced with SOLiD 4 to a read depth of 3-fold nucleotide coverage and 80-fold clone coverage on average.
Extracted DNA was sequenced by SOLiD technology and reads were assigned to species by mapping them to the reference microbial genomes and to the genome of Bos taurus (see Methods section).
The library was sequenced by SOLiD 5500xl sequencer.
The fragment library prepared above was sequenced by SOLiD 5500xl for 7 ligation cycles (X 5 primers) to obtain 35 bp sequences.
Each pool was sequenced in a SOLiD FlowChip on the SOLiD 5500 (Life Technologies, CA) according to the manufacturer's instructions.
Basmati370 rice DNA was sequenced on SOLiD 4 using mate pair library kit with the insert size of 1.5 kb to 2.5 kb.
Briefly, 3 μg genomic DNA from clone 6 was sequenced using SOLiD 5500xl (Life Technologies) mate-pair reads.
A total of 124 samples from leaves, roots and berries were sequenced using SOLiD technology producing approximately 6 billion of directional 75/35 bases paired-end RNAseq reads.
The six new DNA pools were sequenced using SOLiD technology 4, with around 90 million single reads in each pool of 50 nucleotide length.
Three DNA samples from cheeses were sequenced using SOLiD technology, which yielded between 11 and 19 million single reads of 50 nucleotides length.
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