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Differing fragments can then be sequenced to identify specific mutations.
This means that many tumor genomes will need to be sequenced to identify true tumor-driving mutations.
Although we excluded recently vaccinated children in whom vaccine virus was detected, all detected viruses had to be sequenced to identify children who were shedding vaccine virus.
The HRM assay is useful as a pre-screening test and positive samples should be sequenced to identify the specific nucleotide alterations.
An abnormality in either the abundance of protein production or its molecular mass may be because of mutations in the corresponding gene, which could then be sequenced to identify the corresponding nucleotide sequence variant.
However, because the mutant alleles of pSNMs are often present in only a small subpopulation of cells, a large number of cells would need to be sequenced to identify them.
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The gtsB locus of independent white colonies was sequenced to identify clones with the desired SNPs.
The 5′-RACE products were cloned into the pGM-T vector (Tiangen, Beijing, China) and ten clones were sequenced to identify the transcription start site of pfk-pyk operon.
Control uninfected mouse DNA and human DNAs were sequenced to identify any PCR artifacts that might be attributable to cross-contamination of reagents/adjacent wells (which would be found in human DNA) or mispriming events on the mouse genome.
Two positive clones were sequenced to identify ORFs based on similarities to known proteins, and to ESTs using bioinformatics, and manually by a curator.
Denaturing gradient gel electrophoresis (DGGE) was carried out for the various samples of firewater system (FWS) and the high intensity bands were sequenced to identify the predominant bacteria.
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