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"For the first time this will enable large numbers of patients to be sequenced to get to the bottom of thousands of genetically controlled diseases," says Dramanac, who is also the lead author of the Science paper.
For some colonies (e.g. Colony 1, 25, 31, 49; Figure 4) Symbiodinium ITS2 diversity can be captured with <10 clone sequences, while for others (e.g. Colony 9 and 44) a higher number of clones need to be sequenced to get an accurate estimation of endosymbiont ITS2 diversity.
Altogether, the results clearly point out that other genomes, with an emphasis on elite inoculant strains, still have to be sequenced to get a better understanding of the molecular basis of the "perfect symbiosis" involving soybean and Bradyrhizobium[ 1].
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At least ten cDNA clones of each distinct group were sequenced to get precise information on their potential functions.
For practical manipulations, each concatemer is cloned into a suitable vector, and thousands of such clones (SAGE library) are sequenced to get a profile of the expression pattern of the transcriptome of a given tissue of interest.
Representatives of each OTU were reverse sequenced to get a full-length read.
The partial S-gene fragments were directly sequenced to get information related to the prevailing CCHFV genotypes and their molecular epidemiology in Pakistan.
Whilst the first genome to be sequenced cost many billions, you can get one sequenced today for less than $1,000.
For flexibility and extensibility reasons, the process is composed of a set of steps, which must be performed in sequence to get to an effective analysis (see Figure 9).
And so, I and a lot of you, have done a lot of single gene work where we're using Sanger sequencing to get a single sequence from an amplicon.
The reader is shown enough of the narrative sequence to get an impression of what is going on, and to whet their appetite for more, but much is hidden.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com