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However, this variability correlated with neither the strain origin nor the gene-based phylogeny, so numerous strains from the different ecotypes will need to be sequenced to characterize the lactococcal pan-genome.
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RT-PCR products were sequenced to characterize the HAstV and NLV strains present.
Approximately 15 different clones from each PCR product were sequenced to characterize the methylation status of the CpGs.
Flies were made isogenic at the third chromosome using balancers and a ∼1 kb region of mth was sequenced to characterize each line by haplotype as determined by SNP identity [24], [25].
For example, many inbred mouse strains have been sequenced to characterize their genomic variation (Keane et al., 2011).
meliloti had been sequenced to characterize each bacterial isolate (DNA extraction and sequencing procedures are described in an additional file [see Additional file 3]).
We designed a real-time reverse transcription PCR (rRT-PCR) to rapidly identify PDCoV, and 4 additional PDCoV strains were sequenced to further characterize PDCoV in the United States.
Sixteen BAC clones from the Mexican axolotl (Ambystoma mexicanum: c-value = 32 × 109 bp) were isolated and sequenced to characterize the structure of genic regions.
A recent study revealed that millions of transcript tags have to be sequenced in order to fully characterize a human transcriptome ([ 8- 10]).
The heterodimeric porcine umami receptor (T1r1/T1r3) was the first porcine TR to be sequenced, cloned and fully characterized [ 29– 31].
A set of 15,000 clones is also being sequenced (both ends) to characterize this second library.
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