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Short-read sequencers can yield tens of millions of reads in a single run, allowing many targets to be sequenced simultaneously as long as the coverage is deep enough for analysis.
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In fact, 2 to 96 different samples can be sequenced simultaneously using commercial barcoding kits, such as Ion Xpress Barcode kit (Life Technologies) and Nextera XT Index kit (Illumina).
Moreover, a number of different samples, which can be distinguished by barcoding, may be sequenced simultaneously.
Since the endosymbiont is an obligate symbiont, the nematode genome and bacterial genome were sequenced simultaneously.
DNA was sequenced as previously described [24].
Thirteen small RNA libraries were constructed and sequenced simultaneously.
This is a result of the elimination of stray light effects present when multiple sequences are synthesized simultaneously as well as by the intrinsically low rate of depurination and other side reactions in the MAS chemistry, which does not use acidic conditions and requires minimal exposure to oxidants.
As discussed in Results it is beneficial to have seven or eight related strains sequenced simultaneously rather than comparing a single mutant to a parent.
Multiple samples can also be simultaneously sequenced by barcoding (multiplexing).
An adjacent lake was monitored simultaneously as a reference ecosystem.
GAPDH was performed simultaneously as a standard internal control.
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