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"Gordon Moore worked out all the tricks that gave us modern semiconductors, so he should be the first person to be sequenced on a semiconductor," Dr. Rothberg said.
The first domain relates to one-machine job sequencing problems in which a set of jobs must be sequenced on a machine in such a way that a penalty function is minimized.
With the current capacity, an entire genome can be sequenced on a single flow cell by making pools of large insert clones and subsequently multiplexing these pools.
The development of massively-parallel sequencing (MPS) in the last decade has allowed the product of bisulphite conversion to be sequenced on a scale never previously possible.
Finally we developed a custom set of index tags and primers which increase the number of samples that can simultaneously be sequenced on a single lane of an Illumina instrument.
Amplicons were sequenced either in house using a SequiTherm EXCEL™ II DNA Sequencing Kit (Epicentre® Biotechnologies, Madison, WI) on the LI-COR 4300 DNA Analyzer, or sent to the University of Georgia core genomics facility to be sequenced on a 16 capillary ABI 3100.
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The purified DNA was sequenced on a Solexa sequencer (Illumina).
The library was sequenced on a Illumna GAIIx sequencer.
The resulting libraries were sequenced on a gs-flx sequencer using the standard manufacturer's protocol.
Finally, the amplicons were sequenced on a 454 Genome Sequencer (GS) device using FLX Titanium chemistry.
Each cDNA library was sequenced on a Roche 454 sequencer using manufacturer's protocols.
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