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The advent of next-generation technologies (such as the Roche 454 GS FLX+, llumina Hiseq 2000, Applied Biosystems SOLID and HeliScope single-molecule sequencer machines), which allow a human genome to be sequenced in a single week-long run, has led to a large shift in our understanding of the mutations that drive cancers [ 2].
The genomes of microbes can now be sequenced in a matter of weeks, giving new insights into their structure.
For example, small and large RNAs from the same sample cannot be sequenced in a single sequence run.
Increased sample throughput and clever barcoding strategies allow for several sample libraries to be sequenced in a single instrument run2,3,4,5,6.
Using microfluidics of droplet technologies, hundreds to thousands of cells can be sequenced in a single run [41, 42].
But by now a person's entire DNA can be sequenced in a matter of days at a cost of just several hundred pounds.
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All amplified fragments were sequenced in a ABI3730XL automated sequencer.
Additionally, TERTp was sequenced in a separate cohort of 19 patients with radiation-associated meningiomas.
The amplicons were sequenced in a 24-capillary 3500xl Genetic Analyzer (Applied Biosystems).
It's weird to think about episodes of an anthology series being sequenced in a specific way.
Each library was sequenced in a single lane.
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