Sentence examples for be sequenced directly without from inspiring English sources
These PCR fragments can be sequenced directly without the need for cloning PCR fragments first.
An important feature of agt1 in Peperomia is that PCR amplicons typically were resolved as a single sharp target sized band on agarose gels and, for most samples, could be sequenced directly without cloning.
These amplicons were sequenced directly (without cloning) with AB3730 xl 96-capillary sequencers at the Australian Genome Research Facilities AGRFF) in Brisbane, Australia.
PCR products were sequenced directly without cloning.
Purified amplicons were sequenced directly without cloning.
This sample was sequenced directly without barcode, and with 16 barcode pairs.
dicoccum line BS2E, T. durum line Kristall (Vrn-D4), T. turgidum K-16156) were sequenced directly without cloning and contain only one variant of sequence judging from the absence of superpositions in the chromatograms.
It has to be mentioned that, when comparing gonad vs somatic mtDNAs, the different sequencing protocols (from PCR products or cloning, see Methods section for details) might affect our comparisons: actually, given that gonad mtDNAs have been sequenced directly without cloning, low frequency variants would not be detected among them.
All samples were screened and all positive samples were sequenced directly from primary swab material, without prior virus isolation.
