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The amplified products were purified from agarose gel and bound with a pMD18-T vector and then transformed into Escherichia coli DH5 and the recombinant clones with a 1.5 kb insert were sent to be sequenced by sequencing company.
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The resulting libraries were sequenced by Illumina sequencing technology (Hiseq 2000 sequencer; Illumina, San Diego, CA, USA).
The eluted fragment was sequenced by automated sequencing (Eurofins MWG).
The PCR product was sequenced by Sanger sequencing.
Ninety-two LVNC patients and 254 controls were screened and NEXN was sequenced by Sanger sequencing.
The obtained amplicons were sequenced by a commercial sequencing company (Sangon Biotech, Shanghai, China).
The obtained overlapping PCR products were sequenced by BGI tech sequencing service (Beijing, China).
Purified products were sequenced by capillary sequencing.
The SCCmec of the S. epidermidis was sequenced by conventional sequencing of PCR products.
All purified plasmids were sequenced by Sanger sequencing (GATC, Germany).
The other amplicons were sequenced by direct sequencing.
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