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Therefore, not only the high densification of the nanoceramics was confirmed by XCT measurements, but also the two oxide phases of close density and diffusion factors could be separated over a large material volume.
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Immune cells were separated over a two-phase Percoll-density gradient.
Tryptic peptides were separated over a 120 min gradient from 7 to 32% acetonitrile with 0.1% formic acid and measured on a Thermo Q-Exactive mass spectrometer.
When intracellular membranes were separated over a linear sucrose gradient, MYOC equilibrated in a fraction less dense than traditional secretory vesicles and lysosomes.
I (2 g) was separated over a silica gel column (acetone/MeOH/diethylamine, 40 1 1 10 10 1) and purified by a Sephadex LH-20 column (MeOH) to afford lycodoline (30 mg).
Fraction B (86.4 g) was separated over a MCI-gel column (MeOH-H2O from 8 2 to 10 0) to obtain five fractions (Fr. B1 B5).
B3 (27 g) was separated over a MCI-gel column (MeOH-H2O from 85:15 to 100 0) to obtain six fractions (Fr. B3a B3f).
Fraction B (223 g) was separated over a MCI gel column (MeOH-H2O from 70 30 to 100 0) to obtain eight fractions (Fr. B1 B8).
Fraction C (25.7 g) was separated over a silica gel column eluted with gradient mixtures of petroleum ether acetone (35:1–1:1, v/v) to afford major fractions C1-C6.
The blood was separated over a Histopaque 1083 (Sigma-Aldrich, St . Louis MO) gradient, saving the interface cells.
Proteins were separated over a SDS-PAGE gel, transferred to a PVDF membrane and probed with a specific antibody against the target protein.
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