TSE was previously shown to be sensitive to mutations affecting HP1 since a negative, dose-dependent, effect on TSE was found with two loss of function alleles of Su var)205 (including Su var)2 505) [33].
In line with this, we found several HIV epitopes that are presented by protective HLA molecules to be located at the dimer interface of p24, which is expected to be sensitive to mutations.
FunSeq intersects regions of the genome that are likely to be sensitive to mutations with an analysis for variants that disrupt transcription-factor binding sites (Khurana et al., 2013).
However, the primary sequence of such short RNA thermometers can be sensitive to mutation, as a single base change can render the hairpin inactive in vivo.
Previous studies have shown that kinase ATP site inhibitors may be sensitive to mutation at this position, especially where a small amino acid such as threonine in the case of PfCDPK1 is replaced with one containing a bulkier side chain, while ATP binding is unaffected (22).
Finally, we show that the production of these small RNAs is sensitive to mutations affecting squash and zucchini, as well as to the dose of HP1.
Further, both TSE and the repression established by telomeric P elements are sensitive to mutations affecting HP1 and the piwi-family protein AUBERGINE [25], [30], [31].
VHH RSV-D3 and RSV-C4 were found to be sensitive to typical mutations in antigenic site II, confirming the competition pattern with Synagis, while VHH RSV-E4 was sensitive to mutations in the antigenic site IV VI, confirming the competition results with 101F (Fig. 2).
Furthermore, the identification and use of telomeric P elements as P cytotype determinants [2], [16] [18], has made it possible to show that P cytotype (like TSE) involves a strictly-maternally inherited component (called the pre-P cytotype) [67], is sensitive to mutations affecting HP1 [68, [68] and aubergine [1], [69] and is correlated to maternal deposition of piRNAs [70].
A second type of gating is thought to occur by a sort of constriction of the selectivity filter, first recognized in the form of C-type inactivation [33], and is sensitive to mutations at residues neighboring the external entrance of the pore.
Finally, this repression is sensitive to mutations affecting aubergine, a key player of the piRNA pathway.
