Moreover, while we had chosen 10µM IDV for our proof-of-concept experiments, the assay was clearly shown to be sensitive at lower concentrations as well.
In contrast, the developing parasite population is sensitive, at similar concentrations, to anti-liver stage antimalarials (atovaquone and KAF156) that block P. yoelii from growing in hepatocytes.
Results showed that the biosensor was sensitive at a concentration of 103 cell culture infective dose per milliliter (CCID/ml) of BVDV antigens.
It is noteworthy that B. cereus was most sensitive of the test bacteria to the ethanol extract, being sensitive at a concentration of 50 mg/ml.
The principle governing the use of antibiotics is to ensure that the patient receives one to which the target bacterium is sensitive, at a high enough concentration to be effective but not cause side effects, and for a sufficient length of time to ensure that the infection is totally eradicated.
Seven of the 10 EGFR mutant lines were sensitive at a clinically achievable concentration (<1 µM).
Our data therefore suggest that in rodents, adaptive immune functions may be sensitive to PFOA at concentrations ≥ 3.75 mg/kg/day, a dose that resulted in a serum concentration of 7.5 × 10 ng/mL.
Surprisingly, none of the MPM cell lines were sensitive to AT-IAP at concentrations up to 10 μM, either alone, or in cells cocultured with TNF α.
Similar to Kv4 channels, Kv1.5 is also known to be sensitive to flecainide at low micromolar concentrations (16), and application of 20 μM flecainide inhibited the A-type K+ current in retinal arterioles by >80%.
For PCT to be a useful assay to exclude infection and reduce antibiotic use, results must be readily available to the clinician for decision making, it must be sensitive and well validated at low concentrations, and it must be cost-effective, taking into account the equipment cost and day-to-day costs in the laboratory.
