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Initially, we screened each protein in a small number of independent samples from three pre-malignant and three malignant subjects, seeking to confirm our proteomic results and determine those showing the most significant and consistent quantitative differences, which would then be selected for validation in a larger number of patient samples.
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MODIS image of the same date has been selected for validation.
The most effective method was selected for validation in fortification studies with GC-ECD analysis.
Eight proteins were selected for validation in GC patient sera versus healthy controls.
Two transcription factors identified as putative OsSMF1 target genes, RPBF and ONAC024, were selected for validation of their expression in the OsSMF1-transformed calli by qPCR.
Five novel microRNAs were selected for validation.
Some of these proteins were selected for validation.
106 SNPs and 30 indels detected in this study were selected for validation by Sanger sequencing.
Ten genes were selected for validation by quantitative real-time RT-PCR (qPCR).
Large contig sequences from the two air libraries were selected for validation by semi-quantitative PCR.
Nine genes of functional interest were selected for validation by quantitative PCR (qPCR) analysis (Table 2).
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