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Although a large number of SNP genotyping platforms are available, depending on the need and requirements, most suitable platform can be selected for using the SNPs in pigeonpea genetics and breeding applications.
Following I-SceI induction, inaccurate DSB repair generates HPRT-negative mutants, which can be selected for using 6-thioguanine (6-TG), and indels can be detected by PCR amplification using primers flanking the break site.
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Based on the results of the stage 1 survey, the participants were selected for using the mobile and Ubiquitous Learning Log (ULL) system.
The first crossover event was selected for using streptomycin, and the second with 5% sucrose.
The three constructs were separately transfected into mouse ES cells and random integration events were selected for using G418.
Cells were transfected as described above, except after 24 h transfection, cells containing vectors were selected for using media containing 400 µg/ml geneticin (Sigma).
The parasites containing PfSBP1CAD- and SOLCAD-plasmids were selected for using 5 nM WR99210.
Three days post-infection, the infected cells were selected for using puromycin (2.5 µg/ml, MP Biochem).
Poly-A RNA was selected for using the MicroPoly(A) Purist kit (AM1919; Ambion).
Coupled with a NahR reporter system, catalytically active enzyme was selected for using a tet-based host [ 130].
Canavanine resistance was selected for using plates supplemented with 60 μg/mL canavanine (Sigma-Aldrich, St . Louis MO).
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