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All contig sequences with their RD and GR values can be downloaded from our website; alternatively, the contigs can be selected by sequence similarity searches against user-provided queries [ 51].
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Operational Taxonomic Units (OTUs) were selected by using sequences with a 3% dissimilarity level.
The annealed oligos were cloned into the DR274 plasmid between the BsaI restriction sites and transformed into bacteria, which was selected by standard sequencing.
To improve the resolution of the image in this case study, the highest level is selected by clicking the sequence "Graphic settings" → "Interpolation level" → "High" (slides 10 and 11).
The corresponding region of C. reinhardtii D1 protein (residues 199 292) was selected by structure-based sequence alignment obtained by superimposing the three dimensional structures of R. sphaeroides L (PDB code 2BOZ) [57] and Termosynechococcus elongatus D1 (PDB code 2AXT) [2AXT
The actins from chordates, invertebrates, plant and yeast were selected by using the sequence most similar to the alpha-skeletal actin isoform.
The specific sequences were selected by alignment of all sequences available for each virus from the National Center for Biotechnology Information (NCBI) GenBank Database.
For unclassified consensus sequences, the longest representative sequences were selected by comparing the corresponding genome sequences using the CLUSTALW multiple alignment program.
The cDNA library clones were selected by PCR fragments, sequenced and analyzed via Phred, CAP3 and algorithms described in methods.
Testis-specific genes were selected by using only sequences from libraries where the tissue type included the keyword "testis" and not the keyword "embryo".
Since all cocoa GPAT sequences were distinct from the two S. cerevisiae GPAT sequence of SCT1 and GPT2, the potential cocoa CB biosynthetic GPAT sequences were selected by comparison with the GPAT sequences of A. thaliana (Fig. 3a).
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