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Intracellular antibodies (intrabodies) are single-chain (scFv) and single-domain (dAb; nanobody) variable fragments that can retain the affinity and specificity of full-length antibodies, but can be selected and engineered as genes.
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In this chapter, we review the principal mouse DS models which have been selected and engineered over the years either for large genomic regions or for a few or a single gene of interest.
Ab fragments and IgGs are selected and engineered in E. coli and this microorganism may be also an ideal vector for their intracellular delivery.
Some specialized data, such as heat transfer coefficients, interface thermal resistances and system flow resistances, are selected and calculated by thermal engineers.
Finally, the optimal solutions were selected and proposed for use by thermal engineers.
Several hapten-specific antibodies, which were successfully selected and engineered to high specificity and affinity using display technologies, have been found to be amenable to conventional immunoassay development.
In the present work, by applying the PoPMuSiC algorithm for the calculation of the folding free energy, Lys114 of a Bacillus catalase was rationally selected and engineered to improve the thermostability.
Microalgal strains and growing conditions can be selected or engineered to match varying climatic conditions and commodity demands, resulting in algal biomass highly enriched in proteins, carbohydrates, or lipids (typically 30%% and up to 60 75 % by dry weight), depending on its use [12].
Microalgal strains and growing conditions can be selected or engineered to match varying climatic conditions and commodity demands, resulting in algal biomass highly enriched in proteins, carbohydrates, or lipids (typically 30%% and up to 60 75 % by dry weight), depending on its use [ 12].
Based on these findings, we suggest that human IgG mAbs that target β2M for cancer immunotherapy be selected or engineered so as not to activate complement, thus eliminating the potential adverse effects of complement-mediated lysis of normal cells.
Alternatively, microbial fermentation hosts may be selected or engineered to tolerate or even metabolize the toxic compounds from lignocellulose hydrolysates.
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