The exponential distribution is highly right skew, far more non-normal than would be seen in any real microarray experiment.
These ORFs, the number of clones in each ORF that was included on the microarray, and the corresponding average signal ratios can be seen in Table S9.
It can be seen in Table 2 that the sscDNA yield from 50 ng of HUVEC RNA was in excess of the 5 µg required for microarray hybridisations.
However, we have no indications that ASHH2 works directly through SPL/NZZ as no down-regulation of this gene was seen in our microarray experiment, and locule development and meiosis occur, albeit always producing a reduced number of microspores (Figures 4A and 4B).
Repressed genes also had the same trend for the ISH analysis as was seen in the microarray analysis.
Increased gene expression of the transferases is seen in the microarray data in the gut of an E isolate-infected mouse compared to an S isolate-infected gut.
Differential expression of cell cycle regulators, genes encoding signal transduction molecules, transcription factors, and DNA repair enzymes has been seen in multiple microarray experiments [ 10].
As shown in Figure 7A, each probe showed the same trend for the ISH analysis as was seen in the microarray analysis, namely a significant increase in expression of each gene in cells infected with bacteria as indicated by a positive, brown staining reaction.
A strong CD105+/CD71+ signature was seen in the microarray data, and although genes associated with this array signature were assessed by qRT-PCR (e.g. C5orf4), they were not chosen during model development; it is possible that other candidates from this group could add to algorithm performance.
The connection between fruit development and ripening and the development of the vascular system was seen in a similar microarray study of fruit-ripening in strawberry [ 29].
