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The mutation could not be screened in additional family members to further elucidate the role of this mutation.
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Amplified phages were screened in additional rounds and then plated to obtain isolated clones.
Four rare missense alterations and one of the intronic changes, ATMivs10 -6T>G, a known A-T mutation leading to skipping of the exon eleven in the transcript [ 20], were screened in additional series of 237 familial breast cancer cases and 237 healthy controls.
Exons 5 (where the common H315H polymorphism is located) and 6 of CLU (where a non-synonymous change, p.R338Q, was found in a patient and was absent from controls) were screened in an additional set of 200 control individuals from Portugal.
A total of 43 accessions including positive and negative controls were screened in 2010 (Additional file 6: Table S6).
Mutations of 2010 genes identified by WGS were screened in 150 additional NSCLCs by exome captured and sequencing.
To compare SPF expression across tissues, the housekeeping gene Elongation Factor -1α (EF-1α) was screened in parallel (Additional file 1) [ 31].
However, in outbreaks, when mild or asymptomatic persons were screened in Bangladesh, few additional Nipah cases were identified (J. Hossain, pers. comm).
In total, 81 mice were screened in this study (see Additional file 1: Table S1).
The secondary conditions detectable by a multiplex technology such as MS/MS or HPLC and included in a differential diagnosis for the primary target conditions can be screened at minimal additional cost and are, in fact, determined in the diagnostic setting during follow-up.
It is estimated that to prevent one prostate cancer-specific death, 1400 men need to be screened, resulting in approximately 50 additional cases of unnecessarily diagnosed cancer [51].
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