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Location and consolidation of the tubercles will be scored in duplicate by two experienced surgeons (DDH and NWLS).
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All behaviors were scored in duplicate with the aid of computer-assisted analysis software (ButtonBox v.5.0, Behavioral Research Solutions, Madison, WI, USA), once during the experiment and once during a subsequent 'off-line' analysis session by a different observer that was blind to the age and gender of the interacting mice.
Data was extracted and quality was scored in duplicate.
Genotypes were scored in duplicate and checked against pedigree information.
Twenty-five cells were scored in duplicate per dog-whelk (50 in total).
Two-hybrid interactions of serially diluted cells were scored in duplicate on dropout media lacking adenine.
Twenty-five percent of all CPP tests (149 of 596 mice) were scored in duplicate and interrater reliabilities were high for each experiment (Pearson's correlation > 0.99 for each test).
Microsatellite genotypes for families F12, F20, and F45 were scored in duplicate reactions for at least 5% of individuals and an average of 17 markers, in each family (L. V. Plough, G. Shin, and D. Hedgecock, unpublished data), revealing an average error rate of 2.5%.
Samples were scored from duplicate tissue culture wells.
The counts were scored blindly and counted in duplicate slides.
In most experiments more than 25 worms were scored and experiments were performed in duplicate or triplicate.
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