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Due to the increased marker diversity of the YRI cohort compared to the CEU, a greater number of individuals need to be sampled for complete marker saturation.
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Blood was sampled for complete blood cell count and plasma fibrinogen analysis.
Each taxon sampled by Hua et al. [ 28] was sampled for complete mitochondrial genomes, so the number of characters available for phylogenetic inference was large.
After admission, blood was sampled for complete blood count (CBC) with differential count (DC) analysis, biochemistry, glucose levels, and blood culture.
Out of about 2000 samples that had been screened previously for haplogroup-diagnostic RFLP markers and subjected to control region sequencing [25] [30], a total of 113 samples representing subclusters U5a (79 samples) and U5b (34 samples) were selected for complete mtDNA sequencing (Table S3).
Out of 1751 M samples, 750 samples were selected for complete mtDNA sequencing.
Out of about 4500 samples that had been screened previously for haplogroup-diagnostic RFLP markers and subjected to control region sequencing [10], [27], [41] [49], a total of 182 samples representing haplogroups C (83 samples) and D (99 samples) were selected for complete mtDNA sequencing (Table S6).
Samples were analyzed for complete serological tests (surface and core antigen) and PCR were performed on serum samples and DBS.
In order to remove moisture from the sample, the prepared samples were heated at 100° C in the oven for 1 h, then the samples were grinded for complete homogeneity and then passed through 63 μm sieve (ASTM # 230).
Additional file 6: List of population samples subjected previously for haplogroup-diagnostic RFLP screening and control region sequencing from where 166 samples were selected for complete mtDNA sequencing.
Further, 20 Native American and 7 Asian samples were selected for complete sequencing of mtDNA genomes.
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